RESEARCH ARTICLE
The Mixture of Topical Anesthetic and Chlorhexidine at the Delivery Site in Microbial Reduction Efficiency Alternate Title: The Mixture of Topical Anesthetic
Ratchapin Laovanitch Srisatjaluk1, *, Thanawat Kaewjiaranai1, Natthamet Wongsirichat2, 3, **
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
First Page: 114
Last Page: 120
Publisher ID: TOATJ-19101602
DOI: 10.2174/2589645801913010114
Article History:
Received Date: 28/07/2019Revision Received Date: 09/09/2019
Acceptance Date: 15/10/2019
Electronic publication date: 15/11/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Antimicrobial properties of some anesthetic agents have been reported which may be useful for infection control. Topical lidociane (10%) showed some extent of antimicrobial activity on oral microflora.
Objective:
This study aimed to determine whether the antimicrobial efficiency of topical lidocaine can be enhanced by adding chlorhexidine.
Methods:
The Lidocaine-Chlorhexidine (LD-CHX) preparation was prepared by mixing 2.0% CHX and 10% LD with various ratios. The anesthetic efficiency was tested on the oral mucosa of 26 volunteers by Pin Prick Test. Pain scores were recorded using the Numerical Rating Scale (NRS). Antimicrobial effects of 10% LD, 0.2% CHX, and LD-CHX preparations were evaluated. In vitro study was conducted against seven standard bacterial strains. For in vivo study, oral biofilms of 26 volunteers were collected by imprint technique using filter papers. Bacterial growth from the samples after applying the test solutions was compared to the control. Antimicrobial efficiency was expressed as microbial reduction scores from 4-0 (highest to lowest).
Results:
The LD-CHX preparations that had comparable anesthetic efficiency to 10% LD were those containing 9% and 8% LD. These LD-CHX preparations showed microbial reduction scores of 3 and 4 on seven bacterial strains and oral biofilms.
Conclusion:
This study showed the antimicrobial and anesthetic effectiveness of new lidocaine-chlorhexidine preparations both in vitro and in vivo. We also confirmed the effectiveness of infection control protocol in oral surgery using CHX mouthwash prior to topical LD.